One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm
Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype.Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P.aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid P.aeruginosa biofilms is an att